DBNascent | Samples

SRR5034773 Sample ID 1927

**Data sources**
Paper identifier	Ngoc2017human
SRP accession	SRP093742
GEO accession	GSE90035
Samples	SRR5034773

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	44 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	5'GRO-seq without RNA fragmentation
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	75
Read depth after trimming	20,150,474
Duplication proportion	0.73617
Proportion reads mapped	0.8678
Exon/intron ratio	5.69805
Unique read proportion	0.30879

**Bidirectionals**
Total # Tfit bidirectionals	32595
# Promoter Tfit bidirectionals	9500
# Intronic Tfit bidirectionals	13543
# Intergenic Tfit bidirectionals	9670
Total # dREG bidirectionals	29946
# Promoter dREG bidirectionals	10525
# Intronic dREG bidirectionals	11541
# Intergenic dREG bidirectionals	8753
Part of Tfit master merge?	True
Part of dREG master merge?	True