DBNascent | Samples

SRR513507 Sample ID 861

**Data sources**
Paper identifier	Fuda2012fcp1
SRP accession	SRP013761
GEO accession	GSE38748
Samples	SRR513507

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	18 Mb
Stranded gene read counts	TXT file	707 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	cell line
Cell type	S2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	dsRNA LacZ (10 microg/mL)	-5 day	0 day	5 day

**Extended notes**
Pipeline info and software versions
Sample notes	GEO protocols consistent with GRO-seq, and data included in paper, but no mention of protocol in paper
Analysis notes

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	59
Read depth after trimming	2,448,283
Duplication proportion	0.31512
Proportion reads mapped	0.5127
Exon/intron ratio	1.22455
Unique read proportion	0.45361

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False