DBNascent | Samples

SRR513509 Sample ID 863

**Data sources**
Paper identifier	Fuda2012fcp1
SRP accession	SRP013761
GEO accession	GSE38748
Samples	SRR513509

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	17 Mb
Stranded gene read counts	TXT file	706 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	cell line
Cell type	S2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	dsRNA Fcp1 (10 microg/mL)	-5 day	0 day	5 day

**Extended notes**
Pipeline info and software versions
Sample notes	GEO protocols consistent with GRO-seq, and data included in paper, but no mention of protocol in paper
Analysis notes

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	59
Read depth after trimming	1,898,176
Duplication proportion	0.30228
Proportion reads mapped	0.5381
Exon/intron ratio	1.15194
Unique read proportion	0.4459

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False