DBNascent | Samples

SRR5144955 Sample ID 2850

**Data sources**
Paper identifier	Zhu2017comprehensive
SRP accession	SRP096029
GEO accession	GSE93127
Samples	SRR5144955

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	898 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	182 Kb
dREG bidirectional regions	BED file	39 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ECOMG
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol withdrawal	-5 day	0 day	5 day

**Extended notes**
Pipeline info and software versions
Sample notes	Pluripotency maintained with 1 microM 17-beta-estradiol, differentiation to neutrophils induced by withdrawal
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	50
Read depth after trimming	12,560,915
Duplication proportion	0.76894
Proportion reads mapped	0.6054
Exon/intron ratio	2.897
Unique read proportion	0.22728

**Bidirectionals**
Total # Tfit bidirectionals	2142
# Promoter Tfit bidirectionals	397
# Intronic Tfit bidirectionals	1028
# Intergenic Tfit bidirectionals	178
Total # dREG bidirectionals	919
# Promoter dREG bidirectionals	180
# Intronic dREG bidirectionals	336
# Intergenic dREG bidirectionals	314
Part of Tfit master merge?	True
Part of dREG master merge?	True