DBNascent | Samples

SRR5144956 Sample ID 2851

**Data sources**
Paper identifier	Zhu2017comprehensive
SRP accession	SRP096029
GEO accession	GSE93127
Samples	SRR5144956

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	28 Mb
Stranded gene read counts	TXT file	903 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	381 Kb
dREG bidirectional regions	BED file	82 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ECOMG
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol withdrawal	-5 day	0 day	5 day

**Extended notes**
Pipeline info and software versions
Sample notes	Pluripotency maintained with 1 microM 17-beta-estradiol, differentiation to neutrophils induced by withdrawal
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	37,889,429
Duplication proportion	0.86884
Proportion reads mapped	0.6871
Exon/intron ratio	2.51627
Unique read proportion	0.14165

**Bidirectionals**
Total # Tfit bidirectionals	4495
# Promoter Tfit bidirectionals	915
# Intronic Tfit bidirectionals	2321
# Intergenic Tfit bidirectionals	308
Total # dREG bidirectionals	1901
# Promoter dREG bidirectionals	515
# Intronic dREG bidirectionals	653
# Intergenic dREG bidirectionals	495
Part of Tfit master merge?	True
Part of dREG master merge?	True