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SRR5144958 Sample ID 2853

**Data sources**
Paper identifier	Zhu2017comprehensive
SRP accession	SRP096029
GEO accession	GSE93127
Samples	SRR5144958

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	50 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	575 Kb
dREG bidirectional regions	BED file	211 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ECOMG
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Pluripotency maintained with 1 microM 17-beta-estradiol, differentiation to neutrophils induced by withdrawal
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	33,766,468
Duplication proportion	0.65891
Proportion reads mapped	0.6833
Exon/intron ratio	3.77107
Unique read proportion	0.37092

**Bidirectionals**
Total # Tfit bidirectionals	6760
# Promoter Tfit bidirectionals	1791
# Intronic Tfit bidirectionals	3348
# Intergenic Tfit bidirectionals	411
Total # dREG bidirectionals	4886
# Promoter dREG bidirectionals	1520
# Intronic dREG bidirectionals	1519
# Intergenic dREG bidirectionals	1210
Part of Tfit master merge?	True
Part of dREG master merge?	True