DBNascent | Samples

SRR5150535 Sample ID 1929

**Data sources**
Paper identifier	Ngoc2017human
SRP accession	SRP093742
GEO accession	GSE90035
Samples	SRR5150535

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	41 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	346 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	GRO-seq control sample; Small RNA Library Prep Set (New England Biolabs) was used for final library preparation with modifications as described in Hetzel et al. 2016
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	75
Read depth after trimming	8,256,611
Duplication proportion	0.2954
Proportion reads mapped	0.8978
Exon/intron ratio	2.4767
Unique read proportion	0.66921

**Bidirectionals**
Total # Tfit bidirectionals	15920
# Promoter Tfit bidirectionals	5955
# Intronic Tfit bidirectionals	6682
# Intergenic Tfit bidirectionals	2544
Total # dREG bidirectionals	8045
# Promoter dREG bidirectionals	4879
# Intronic dREG bidirectionals	2255
# Intergenic dREG bidirectionals	1102
Part of Tfit master merge?	True
Part of dREG master merge?	True