DBNascent | Samples

SRR5195273 Sample ID 16

**Data sources**
Paper identifier	Agarwal2021kdm1a
SRP accession	SRP097614
GEO accession	GSE93938
Samples	SRR5195273

**Technical details**
QC score	1
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	107 Mb
Stranded gene read counts	TXT file	922 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	623 Kb
dREG bidirectional regions	BED file	332 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	shRNA control (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-32 min	0 min	32 min

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	51
Read depth after trimming	36,853,477
Duplication proportion	0.26983
Proportion reads mapped	0.7509
Exon/intron ratio	1.80575
Unique read proportion	0.77041

**Bidirectionals**
Total # Tfit bidirectionals	7291
# Promoter Tfit bidirectionals	3137
# Intronic Tfit bidirectionals	2437
# Intergenic Tfit bidirectionals	339
Total # dREG bidirectionals	7699
# Promoter dREG bidirectionals	3381
# Intronic dREG bidirectionals	1945
# Intergenic dREG bidirectionals	1086
Part of Tfit master merge?	True
Part of dREG master merge?	True