DBNascent | Samples

SRR5195275 Sample ID 18

**Data sources**
Paper identifier	Agarwal2021kdm1a
SRP accession	SRP097614
GEO accession	GSE93938
Samples	SRR5195275

**Technical details**
QC score	1
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	99 Mb
Stranded gene read counts	TXT file	922 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	613 Kb
dREG bidirectional regions	BED file	315 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	shRNA Lsd1 A (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-32 min	0 min	32 min

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	51
Read depth after trimming	34,671,823
Duplication proportion	0.24511
Proportion reads mapped	0.7091
Exon/intron ratio	1.82562
Unique read proportion	0.78603

**Bidirectionals**
Total # Tfit bidirectionals	7174
# Promoter Tfit bidirectionals	2969
# Intronic Tfit bidirectionals	2410
# Intergenic Tfit bidirectionals	360
Total # dREG bidirectionals	7288
# Promoter dREG bidirectionals	3217
# Intronic dREG bidirectionals	1779
# Intergenic dREG bidirectionals	1028
Part of Tfit master merge?	True
Part of dREG master merge?	True