DBNascent | Samples

SRR5195277 Sample ID 20

**Data sources**
Paper identifier	Agarwal2021kdm1a
SRP accession	SRP097614
GEO accession	GSE93938
Samples	SRR5195277

**Technical details**
QC score	1
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	120 Mb
Stranded gene read counts	TXT file	923 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	609 Kb
dREG bidirectional regions	BED file	381 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	cortical neuron
Clone/Individual
Strain
Genotype	shRNA Lsd1 B (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-32 min	0 min	32 min

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	51
Read depth after trimming	41,242,242
Duplication proportion	0.24707
Proportion reads mapped	0.7245
Exon/intron ratio	2.02877
Unique read proportion	0.79684

**Bidirectionals**
Total # Tfit bidirectionals	7096
# Promoter Tfit bidirectionals	2771
# Intronic Tfit bidirectionals	2419
# Intergenic Tfit bidirectionals	481
Total # dREG bidirectionals	8835
# Promoter dREG bidirectionals	3514
# Intronic dREG bidirectionals	2531
# Intergenic dREG bidirectionals	1456
Part of Tfit master merge?	True
Part of dREG master merge?	True