DBNascent | Samples

SRR5227983 Sample ID 1654

**Data sources**
Paper identifier	Liu2017transcriptional
SRP accession	SRP098814
GEO accession	GSE94479
Samples	SRR5227983

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	224 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	756 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	nocodazole (200 ng/mL)	-12 hr	0 hr	12 hr
treatment	thymidine (2 mM)	-39 hr	-15 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	M phase
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	49
Read depth after trimming	29,306,292
Duplication proportion	0.08481
Proportion reads mapped	0.953
Exon/intron ratio	2.09923
Unique read proportion	0.808

**Bidirectionals**
Total # Tfit bidirectionals	30863
# Promoter Tfit bidirectionals	9396
# Intronic Tfit bidirectionals	14355
# Intergenic Tfit bidirectionals	5632
Total # dREG bidirectionals	17400
# Promoter dREG bidirectionals	5587
# Intronic dREG bidirectionals	7429
# Intergenic dREG bidirectionals	3720
Part of Tfit master merge?	True
Part of dREG master merge?	True