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SRR5274987 Sample ID 1897
| Paper identifier | Murakami2017dynamic |
| SRP accession | SRP100437 |
| GEO accession | GSE95122 |
| Samples | SRR5274987 |
| QC score | 3 |
| Replicate | 2 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | MDA-MB-231 |
| Clone/Individual | |
| Strain | |
| Genotype | Esr1 L540Q (stable) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 17-beta-estradiol (100 nM) | -20 min | 0 min | 20 min |
| Pipeline info and software versions | |
| Sample notes | L540Q mutant cannot bind SRCs proteins |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 3 |
| Sample NRO score | 2 |
| Raw read length | 51 |
| Read depth after trimming | 18,882,091 |
| Duplication proportion | 0.41899 |
| Proportion reads mapped | 0.5894 |
| Exon/intron ratio | 3.32012 |
| Unique read proportion | 0.58088 |
| Total # Tfit bidirectionals | 25666 |
| # Promoter Tfit bidirectionals | 9747 |
| # Intronic Tfit bidirectionals | 10765 |
| # Intergenic Tfit bidirectionals | 5403 |
| Total # dREG bidirectionals | 18520 |
| # Promoter dREG bidirectionals | 8226 |
| # Intronic dREG bidirectionals | 6975 |
| # Intergenic dREG bidirectionals | 3611 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |