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SRR5274989 Sample ID 1899
| Paper identifier | Murakami2017dynamic |
| SRP accession | SRP100437 |
| GEO accession | GSE95122 |
| Samples | SRR5274989 |
| QC score | 1 |
| Replicate | 4 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | MDA-MB-231 |
| Clone/Individual | |
| Strain | |
| Genotype | Esr1 L540Q (stable) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 17-beta-estradiol (100 nM) | -20 min | 0 min | 20 min |
| Pipeline info and software versions | |
| Sample notes | L540Q mutant cannot bind SRCs proteins |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 1 |
| Sample NRO score | 2 |
| Raw read length | 51 |
| Read depth after trimming | 29,455,086 |
| Duplication proportion | 0.47174 |
| Proportion reads mapped | 0.5776 |
| Exon/intron ratio | 3.32495 |
| Unique read proportion | 0.55211 |
| Total # Tfit bidirectionals | 30805 |
| # Promoter Tfit bidirectionals | 11803 |
| # Intronic Tfit bidirectionals | 12629 |
| # Intergenic Tfit bidirectionals | 6893 |
| Total # dREG bidirectionals | 22481 |
| # Promoter dREG bidirectionals | 8664 |
| # Intronic dREG bidirectionals | 8991 |
| # Intergenic dREG bidirectionals | 4880 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |