SRR5274990 Sample ID 1900

Data sources
Paper identifier Murakami2017dynamic
SRP accession SRP100437
GEO accession GSE95122
Samples SRR5274990
Technical details
QC score 4
Replicate 1
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse True
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 28 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 1 Mb
dREG bidirectional regions BED file 412 Kb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MDA-MB-231
Clone/Individual
Strain
Genotype Esr1 L540Q (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment 17-beta-estradiol (100 nM) -45 min 0 min 45 min
Extended notes
Pipeline info and software versions
Sample notes L540Q mutant cannot bind SRCs proteins
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 4
Sample NRO score 2
Raw read length 51
Read depth after trimming 22,043,224
Duplication proportion 0.57886
Proportion reads mapped 0.3122
Exon/intron ratio 3.31973
Unique read proportion 0.40616
Bidirectionals
Total # Tfit bidirectionals 13853
# Promoter Tfit bidirectionals 7197
# Intronic Tfit bidirectionals 5065
# Intergenic Tfit bidirectionals 1739
Total # dREG bidirectionals 9589
# Promoter dREG bidirectionals 6266
# Intronic dREG bidirectionals 2642
# Intergenic dREG bidirectionals 998
Part of Tfit master merge? True
Part of dREG master merge? True