DBNascent | Samples

SRR5274991 Sample ID 1901

**Data sources**
Paper identifier	Murakami2017dynamic
SRP accession	SRP100437
GEO accession	GSE95122
Samples	SRR5274991

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	37 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	570 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MDA-MB-231
Clone/Individual
Strain
Genotype	Esr1 L540Q (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-45 min	0 min	45 min

**Extended notes**
Pipeline info and software versions
Sample notes	L540Q mutant cannot bind SRCs proteins
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	17,321,551
Duplication proportion	0.4442
Proportion reads mapped	0.4301
Exon/intron ratio	3.37619
Unique read proportion	0.53449

**Bidirectionals**
Total # Tfit bidirectionals	17759
# Promoter Tfit bidirectionals	8188
# Intronic Tfit bidirectionals	7048
# Intergenic Tfit bidirectionals	2906
Total # dREG bidirectionals	13244
# Promoter dREG bidirectionals	7610
# Intronic dREG bidirectionals	4256
# Intergenic dREG bidirectionals	1803
Part of Tfit master merge?	True
Part of dREG master merge?	True