DBNascent | Samples

SRR5298791 Sample ID 1017

**Data sources**
Paper identifier	Incarnato2017vivo
SRP accession	SRP100931
GEO accession	GSE95567
Samples	SRR5298791

**Technical details**
QC score	5
Replicate	1
Protocol	other
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	Yes

**Downloads**
TDF visualization file	TDF file	5 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	E. coli
Sample type	organism
Cell type	whole bacteria
Clone/Individual
Strain	K-12
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	rnpB SPET-seq (in vitro); This is structural data from in vitro nascent transcription - not useful for comparison purposes
Analysis notes	Unstranded and odd (this sample alone was an in vitro assay and not included in tech reps); picard didn't work

**Sample metrics**
Sample QC score	5
Sample NRO score	5
Raw read length	70
Read depth after trimming	21,012,077
Duplication proportion	0.97332
Proportion reads mapped	0.8914
Exon/intron ratio	17.6135
Unique read proportion	0.02194

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False