DBNascent | Samples

SRR5359619 Sample ID 2086

**Data sources**
Paper identifier	Phanstiel2017static
SRP accession	SRP102154
GEO accession	GSE96800
Samples	SRR5359619

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	22 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	182 Kb
dREG bidirectional regions	BED file	66 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	THP1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	phorbol myristate acetate (100 nM)	-72 hr	0 hr	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Data/protocol not mentioned in paper but grouped with data that were included
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	101
Read depth after trimming	20,795,056
Duplication proportion	0.82705
Proportion reads mapped	0.8477
Exon/intron ratio	3.6345
Unique read proportion	0.09609

**Bidirectionals**
Total # Tfit bidirectionals	2105
# Promoter Tfit bidirectionals	653
# Intronic Tfit bidirectionals	963
# Intergenic Tfit bidirectionals	149
Total # dREG bidirectionals	1535
# Promoter dREG bidirectionals	473
# Intronic dREG bidirectionals	611
# Intergenic dREG bidirectionals	374
Part of Tfit master merge?	False
Part of dREG master merge?	False