DBNascent | Samples

SRR5388630 Sample ID 446

**Data sources**
Paper identifier	Chen2018augmented
SRP accession	SRP102697
GEO accession	GSE97183
Samples	SRR5388630

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	49 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	703 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	dox-inducible shRNA Srsf2 endogenous (stable); dox-inducible Srsf2 P95H exogenous (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	40
Read depth after trimming	28,671,198
Duplication proportion	0.76598
Proportion reads mapped	0.9245
Exon/intron ratio	3.70545
Unique read proportion	0.25328

**Bidirectionals**
Total # Tfit bidirectionals	21180
# Promoter Tfit bidirectionals	12087
# Intronic Tfit bidirectionals	7471
# Intergenic Tfit bidirectionals	2299
Total # dREG bidirectionals	16299
# Promoter dREG bidirectionals	9849
# Intronic dREG bidirectionals	5000
# Intergenic dREG bidirectionals	2040
Part of Tfit master merge?	True
Part of dREG master merge?	True