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SRR5388631 Sample ID 447
| Paper identifier | Chen2018augmented |
| SRP accession | SRP102697 |
| GEO accession | GSE97183 |
| Samples | SRR5388631 |
| QC score | 3 |
| Replicate | 2 |
| Protocol | GRO-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 33 Mb |
| Stranded gene read counts | TXT file | 1 Mb |
| Unstranded bidirectional read counts | TXT file | 21 Mb |
| Tfit bidirectional regions | BED file | 1 Mb |
| dREG bidirectional regions | BED file | 565 Kb |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T |
| Clone/Individual | |
| Strain | |
| Genotype | dox-inducible shRNA Srsf2 endogenous (stable); dox-inducible Srsf2 P95H exogenous (stable) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | doxycycline (1 microg/mL) | -5 hr | 0 hr | 5 hr |
| Pipeline info and software versions | |
| Sample notes | |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 3 |
| Sample NRO score | 2 |
| Raw read length | 40 |
| Read depth after trimming | 15,942,230 |
| Duplication proportion | 0.75585 |
| Proportion reads mapped | 0.9262 |
| Exon/intron ratio | 3.89189 |
| Unique read proportion | 0.25199 |
| Total # Tfit bidirectionals | 14893 |
| # Promoter Tfit bidirectionals | 8790 |
| # Intronic Tfit bidirectionals | 5264 |
| # Intergenic Tfit bidirectionals | 1437 |
| Total # dREG bidirectionals | 13172 |
| # Promoter dREG bidirectionals | 9020 |
| # Intronic dREG bidirectionals | 3433 |
| # Intergenic dREG bidirectionals | 1381 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |