DBNascent | Samples

SRR5388636 Sample ID 452

**Data sources**
Paper identifier	Chen2018augmented
SRP accession	SRP102697
GEO accession	GSE97183
Samples	SRR5388636

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	39 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	532 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	dox-inducible shRNA U2af35 endogenous (stable); dox-inducible U2af35 Q157P exogenous (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	40
Read depth after trimming	19,811,027
Duplication proportion	0.74486
Proportion reads mapped	0.8982
Exon/intron ratio	3.01356
Unique read proportion	0.27001

**Bidirectionals**
Total # Tfit bidirectionals	17947
# Promoter Tfit bidirectionals	10713
# Intronic Tfit bidirectionals	5989
# Intergenic Tfit bidirectionals	1654
Total # dREG bidirectionals	12366
# Promoter dREG bidirectionals	8292
# Intronic dREG bidirectionals	3123
# Intergenic dREG bidirectionals	1351
Part of Tfit master merge?	True
Part of dREG master merge?	True