SRR5388637 Sample ID 453

Data sources
Paper identifier Chen2018augmented
SRP accession SRP102697
GEO accession GSE97183
Samples SRR5388637
Technical details
QC score 3
Replicate 2
Protocol GRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype dox-inducible shRNA U2af35 endogenous (stable); dox-inducible U2af35 Q157P exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment doxycycline (1 microg/mL) -5 hr 0 hr 5 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 1
Raw read length 40
Read depth after trimming 16,844,414
Duplication proportion 0.73175
Proportion reads mapped 0.91
Exon/intron ratio 2.98092
Unique read proportion 0.27824
Bidirectionals
Total # Tfit bidirectionals 15198
# Promoter Tfit bidirectionals 9241
# Intronic Tfit bidirectionals 4946
# Intergenic Tfit bidirectionals 1298
Total # dREG bidirectionals 10664
# Promoter dREG bidirectionals 7464
# Intronic dREG bidirectionals 2456
# Intergenic dREG bidirectionals 1090
Part of Tfit master merge? True
Part of dREG master merge? True