DBNascent | Samples

SRR5388637 Sample ID 453

**Data sources**
Paper identifier	Chen2018augmented
SRP accession	SRP102697
GEO accession	GSE97183
Samples	SRR5388637

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	36 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	458 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	dox-inducible shRNA U2af35 endogenous (stable); dox-inducible U2af35 Q157P exogenous (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	40
Read depth after trimming	16,844,414
Duplication proportion	0.73175
Proportion reads mapped	0.91
Exon/intron ratio	2.98092
Unique read proportion	0.27824

**Bidirectionals**
Total # Tfit bidirectionals	15198
# Promoter Tfit bidirectionals	9241
# Intronic Tfit bidirectionals	4946
# Intergenic Tfit bidirectionals	1298
Total # dREG bidirectionals	10664
# Promoter dREG bidirectionals	7464
# Intronic dREG bidirectionals	2456
# Intergenic dREG bidirectionals	1090
Part of Tfit master merge?	True
Part of dREG master merge?	True