SRR5388642 Sample ID 458

Data sources
Paper identifier Chen2018augmented
SRP accession SRP102697
GEO accession GSE97183
Samples SRR5388642
Technical details
QC score 3
Replicate 1
Protocol GRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype dox-inducible shRNA Srsf2 endogenous (stable); dox-inducible Srsf2 exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment doxycycline (1 microg/mL) -5 hr 0 hr 5 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 40
Read depth after trimming 23,166,908
Duplication proportion 0.75113
Proportion reads mapped 0.8863
Exon/intron ratio 3.67913
Unique read proportion 0.26395
Bidirectionals
Total # Tfit bidirectionals 19153
# Promoter Tfit bidirectionals 11016
# Intronic Tfit bidirectionals 6856
# Intergenic Tfit bidirectionals 1926
Total # dREG bidirectionals 15269
# Promoter dREG bidirectionals 9650
# Intronic dREG bidirectionals 4451
# Intergenic dREG bidirectionals 1767
Part of Tfit master merge? True
Part of dREG master merge? True