SRR5388643 Sample ID 459

Data sources
Paper identifier Chen2018augmented
SRP accession SRP102697
GEO accession GSE97183
Samples SRR5388643
Technical details
QC score 3
Replicate 2
Protocol GRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype dox-inducible shRNA Srsf2 endogenous (stable); dox-inducible Srsf2 exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment doxycycline (1 microg/mL) -5 hr 0 hr 5 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 40
Read depth after trimming 20,820,481
Duplication proportion 0.75395
Proportion reads mapped 0.8998
Exon/intron ratio 3.70486
Unique read proportion 0.25859
Bidirectionals
Total # Tfit bidirectionals 17947
# Promoter Tfit bidirectionals 10500
# Intronic Tfit bidirectionals 6333
# Intergenic Tfit bidirectionals 1767
Total # dREG bidirectionals 14623
# Promoter dREG bidirectionals 9507
# Intronic dREG bidirectionals 4087
# Intergenic dREG bidirectionals 1620
Part of Tfit master merge? True
Part of dREG master merge? True