SRR5388643 Sample ID 459

Data sources
Paper identifier Chen2018augmented
SRP accession SRP102697
GEO accession GSE97183
Samples SRR5388643
Technical details
QC score 3
Replicate 2
Protocol GRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 40 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 2 Mb
dREG bidirectional regions BED file 629 Kb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype dox-inducible shRNA Srsf2 endogenous (stable); dox-inducible Srsf2 exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment doxycycline (1 microg/mL) -5 hr 0 hr 5 hr
Extended notes
Pipeline info and software versions
Sample notes
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 40
Read depth after trimming 20,820,481
Duplication proportion 0.75395
Proportion reads mapped 0.8998
Exon/intron ratio 3.70486
Unique read proportion 0.25859
Bidirectionals
Total # Tfit bidirectionals 17947
# Promoter Tfit bidirectionals 10500
# Intronic Tfit bidirectionals 6333
# Intergenic Tfit bidirectionals 1767
Total # dREG bidirectionals 14623
# Promoter dREG bidirectionals 9507
# Intronic dREG bidirectionals 4087
# Intergenic dREG bidirectionals 1620
Part of Tfit master merge? True
Part of dREG master merge? True