DBNascent | Samples

SRR5508395 Sample ID 1628

**Data sources**
Paper identifier	Liu2017dynamic
SRP accession	SRP106474
GEO accession	GSE98555
Samples	SRR5508395

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	83 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	810 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	hematopoietic progenitor cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	G-CSF (10 ng/mL)	-1 day	0 day	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	other differentiation media changes 3 days before extraction; isolated from peripheral blood of healthy volunteers
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	76
Read depth after trimming	48,942,887
Duplication proportion	0.7249
Proportion reads mapped	0.744
Exon/intron ratio	3.05692
Unique read proportion	0.5379

**Bidirectionals**
Total # Tfit bidirectionals	24082
# Promoter Tfit bidirectionals	6316
# Intronic Tfit bidirectionals	12816
# Intergenic Tfit bidirectionals	3931
Total # dREG bidirectionals	18859
# Promoter dREG bidirectionals	7007
# Intronic dREG bidirectionals	8491
# Intergenic dREG bidirectionals	3331
Part of Tfit master merge?	True
Part of dREG master merge?	True