DBNascent | Samples

SRR5633599 Sample ID 784

**Data sources**
Paper identifier	Fleischer2017dna
SRP accession	SRP108366
GEO accession	GSE99508
Samples	SRR5633599

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	52 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	629 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	estradiol (100 nM)	-40 min	0 min	40 min
treatment	hydroxytamoxifen (1 microM)	-40 min	0 min	40 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	10,937,997
Duplication proportion	0.3078
Proportion reads mapped	0.8966
Exon/intron ratio	2.34447
Unique read proportion	0.69482

**Bidirectionals**
Total # Tfit bidirectionals	21075
# Promoter Tfit bidirectionals	9911
# Intronic Tfit bidirectionals	7991
# Intergenic Tfit bidirectionals	3323
Total # dREG bidirectionals	14561
# Promoter dREG bidirectionals	8142
# Intronic dREG bidirectionals	4896
# Intergenic dREG bidirectionals	1924
Part of Tfit master merge?	True
Part of dREG master merge?	True