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SRR5655673 Sample ID 2443
| Paper identifier | Tastemel2017transcription |
| SRP accession | SRP108750 |
| GEO accession | GSE99747 |
| Samples | SRR5655673 |
| QC score | 3 |
| Replicate | 2 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 87 Mb |
| Stranded gene read counts | TXT file | 927 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 5 Mb |
| dREG bidirectional regions | BED file | 2 Mb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | cell line |
| Cell type | ESC |
| Clone/Individual | E14 |
| Strain | |
| Genotype | Spt5 V1008D |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | CH99021 (3 microM) | None | None | None |
| treatment | PD0325901 (1 microM) | None | None | None |
| treatment | serum-free media | None | None | None |
| Pipeline info and software versions | |
| Sample notes | Treatments for "length of culture", no timing specified; PD0325901=MEK inhibitor; CH99021=Gsk3 inhibitor |
| Analysis notes |
| Sample QC score | 3 |
| Sample NRO score | 3 |
| Raw read length | 76 |
| Read depth after trimming | 44,826,907 |
| Duplication proportion | 0.715 |
| Proportion reads mapped | 0.841 |
| Exon/intron ratio | 5.09048 |
| Unique read proportion | 0.50215 |
| Total # Tfit bidirectionals | 59076 |
| # Promoter Tfit bidirectionals | 15510 |
| # Intronic Tfit bidirectionals | 19246 |
| # Intergenic Tfit bidirectionals | 19911 |
| Total # dREG bidirectionals | 43305 |
| # Promoter dREG bidirectionals | 9303 |
| # Intronic dREG bidirectionals | 14225 |
| # Intergenic dREG bidirectionals | 16472 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |