DBNascent | Samples

SRR5655673 Sample ID 2443

**Data sources**
Paper identifier	Tastemel2017transcription
SRP accession	SRP108750
GEO accession	GSE99747
Samples	SRR5655673

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	87 Mb
Stranded gene read counts	TXT file	927 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	5 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	E14
Strain
Genotype	Spt5 V1008D
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	CH99021 (3 microM)	None	None	None
treatment	PD0325901 (1 microM)	None	None	None
treatment	serum-free media	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Treatments for "length of culture", no timing specified; PD0325901=MEK inhibitor; CH99021=Gsk3 inhibitor
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	76
Read depth after trimming	44,826,907
Duplication proportion	0.715
Proportion reads mapped	0.841
Exon/intron ratio	5.09048
Unique read proportion	0.50215

**Bidirectionals**
Total # Tfit bidirectionals	59076
# Promoter Tfit bidirectionals	15510
# Intronic Tfit bidirectionals	19246
# Intergenic Tfit bidirectionals	19911
Total # dREG bidirectionals	43305
# Promoter dREG bidirectionals	9303
# Intronic dREG bidirectionals	14225
# Intergenic dREG bidirectionals	16472
Part of Tfit master merge?	True
Part of dREG master merge?	True