DBNascent | Samples

SRR5681065 Sample ID 1676

**Data sources**
Paper identifier	Liu2018rna-directed
SRP accession	SRP109129
GEO accession	GSE100010
Samples	SRR5681065

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	71 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	A. thaliana
Sample type	tissue
Cell type	inflorescence and meristem
Clone/Individual
Strain	Col 0
Genotype	Frg1 mut; Frg2 mut
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	dirty data - did fastq-dump manually, force trimmed first 4 bp and bp 60+ prior to running pipeline, ran pipeline on trimmed fastqs (with normal bbduk trimming included); ran tech reps on pre-trimmed fastqs directly

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	56
Read depth after trimming	33,214,417
Duplication proportion	0.62054
Proportion reads mapped	0.8284
Exon/intron ratio	2.62308
Unique read proportion	0.31965

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False