DBNascent | Samples

SRR5714119 Sample ID 556

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession
Samples	SRR5714119

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	32 Mb
Stranded gene read counts	TXT file	916 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	923 Kb
dREG bidirectional regions	BED file	433 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Not in GEO, experiment not specifically mentioned in paper - methods unclear
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	35
Read depth after trimming	65,574,863
Duplication proportion	0.93592
Proportion reads mapped	0.6677
Exon/intron ratio	2.13612
Unique read proportion	0.14894

**Bidirectionals**
Total # Tfit bidirectionals	10799
# Promoter Tfit bidirectionals	4906
# Intronic Tfit bidirectionals	3267
# Intergenic Tfit bidirectionals	718
Total # dREG bidirectionals	10042
# Promoter dREG bidirectionals	5195
# Intronic dREG bidirectionals	2534
# Intergenic dREG bidirectionals	839
Part of Tfit master merge?	True
Part of dREG master merge?	True