DBNascent | Samples

SRR5714120 Sample ID 557

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession
Samples	SRR5714120

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	86 Mb
Stranded gene read counts	TXT file	925 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	878 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/microL)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Not in GEO, experiment not specifically mentioned in paper - methods unclear
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	37
Read depth after trimming	127,668,256
Duplication proportion	0.87425
Proportion reads mapped	0.6774
Exon/intron ratio	2.77968
Unique read proportion	0.30083

**Bidirectionals**
Total # Tfit bidirectionals	22175
# Promoter Tfit bidirectionals	9582
# Intronic Tfit bidirectionals	7279
# Intergenic Tfit bidirectionals	2988
Total # dREG bidirectionals	20182
# Promoter dREG bidirectionals	6435
# Intronic dREG bidirectionals	7377
# Intergenic dREG bidirectionals	4008
Part of Tfit master merge?	True
Part of dREG master merge?	True