DBNascent | Samples

SRR574829 Sample ID 2296

**Data sources**
Paper identifier	Sigova2013divergent
SRP accession	SRP015819
GEO accession	GSE41009
Samples	SRR574829

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	149 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H1
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	RPMI/B27	-25 hr	0 hr	25 hr
treatment	activin (50 ng/mL)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	All cells maintained in mTESR1 medium until transferred to RPMI
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	40
Read depth after trimming	93,472,159
Duplication proportion	0.47381
Proportion reads mapped	0.455
Exon/intron ratio	3.46911
Unique read proportion	0.62293

**Bidirectionals**
Total # Tfit bidirectionals	47862
# Promoter Tfit bidirectionals	17389
# Intronic Tfit bidirectionals	19144
# Intergenic Tfit bidirectionals	10234
Total # dREG bidirectionals	31561
# Promoter dREG bidirectionals	8815
# Intronic dREG bidirectionals	12026
# Intergenic dREG bidirectionals	8665
Part of Tfit master merge?	True
Part of dREG master merge?	True