DBNascent | Samples

SRR574830 Sample ID 2296

**Data sources**
Paper identifier	Sigova2013divergent
SRP	SRP015819
Samples	SRR574830

**Technical details**
QC score	1
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	193 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H1
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	RPMI/B27	-25 hr	0 hr	25 hr
treatment	activin (50 ng/mL)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	All cells maintained in mTESR1 medium until transferred to RPMI
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	40
Read depth after trimming	113,896,447
Duplication proportion	0.48171
Proportion reads mapped	0.5016
Exon/intron ratio	3.10348
Unique read proportion	0.65346

**Bidirectionals**
Total # Tfit bidirectionals	51195
# Promoter Tfit bidirectionals	17965
# Intronic Tfit bidirectionals	20649
# Intergenic Tfit bidirectionals	11335
Total # dREG bidirectionals	34533
# Promoter dREG bidirectionals	8890
# Intronic dREG bidirectionals	13522
# Intergenic dREG bidirectionals	9902
Part of Tfit master merge?	True
Part of dREG master merge?	True