SRR574830 Sample ID 2297

Data sources
Paper identifier Sigova2013divergent
SRP accession SRP015819
GEO accession GSE41009
Samples SRR574830
Technical details
QC score 1
Replicate 2
Protocol GRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse True
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type ESC
Clone/Individual H1
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment RPMI/B27 -25 hr 0 hr 25 hr
treatment activin (50 ng/mL) -1 hr 0 hr 1 hr
Extended notes
Pipeline info and software versions
Sample notes All cells maintained in mTESR1 medium until transferred to RPMI
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 1
Sample NRO score 2
Raw read length 40
Read depth after trimming 113,896,447
Duplication proportion 0.48171
Proportion reads mapped 0.5016
Exon/intron ratio 3.10348
Unique read proportion 0.65346
Bidirectionals
Total # Tfit bidirectionals 51195
# Promoter Tfit bidirectionals 17965
# Intronic Tfit bidirectionals 20649
# Intergenic Tfit bidirectionals 11335
Total # dREG bidirectionals 34533
# Promoter dREG bidirectionals 8890
# Intronic dREG bidirectionals 13522
# Intergenic dREG bidirectionals 9902
Part of Tfit master merge? True
Part of dREG master merge? True