DBNascent | Samples

SRR5905175 Sample ID 335

**Data sources**
Paper identifier	Booth2018cdk9
SRP accession	SRP114939
GEO accession	GSE102308
Samples	SRR5905175

**Technical details**
QC score	3
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	other
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	16 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	S. pombe
Sample type	organism
Cell type	whole yeast
Clone/Individual
Strain	PP20 (cdk9as lsk1as)
Genotype	cdk9T120G::hphMX6 lsk1F353G::kanMX6 leu1-32 ura4-D18 his3-D1 ade6-M210 h+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	3MB-PP1 (10 microM)	-5 min	0 min	5 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	76
Read depth after trimming	14,072,225
Duplication proportion	0.7712
Proportion reads mapped	0.7692
Exon/intron ratio	4.11106
Unique read proportion	0.21858

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False