DBNascent | Samples

SRR5929961 Sample ID 2040

**Data sources**
Paper identifier	Parua2018cdk9-pp1
SRP accession	SRP115327
GEO accession	GSE102590
Samples	SRR5929961

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	S. cerevisiae
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	15 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	S. pombe
Sample type	organism
Cell type	whole yeast
Clone/Individual
Strain	LV7 (cdk9as)
Genotype	cdk9T120G::kanMX6 leu1-32 ura4-D18 his3-D1 ade6-M210 h+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cold stress (18 Celsius)	-15 min	0 min	15 min
treatment	3MB-PP1 (10 microM)	-5 min	0 min	5 min

**Extended notes**
Pipeline info and software versions
Sample notes	cdk9as strain is sensitive to 3MBPP1; dis2-11 strain is cold sensitive; 4 biotins used
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	76
Read depth after trimming	15,281,249
Duplication proportion	0.82075
Proportion reads mapped	0.8284
Exon/intron ratio	4.28365
Unique read proportion	0.18956

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False