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SRR5929977 Sample ID 2056
| Paper identifier | Parua2018cdk9-pp1 |
| SRP accession | SRP115327 |
| GEO accession | GSE102590 |
| Samples | SRR5929977 |
| QC score | 4 |
| Replicate | 2 |
| Protocol | PRO-seq |
| Library prep | ligation |
| Spike-in | S. cerevisiae |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 11 Mb |
| Organism | S. pombe |
| Sample type | organism |
| Cell type | whole yeast |
| Clone/Individual | |
| Strain | PP185 (cdk9as dis2-11) |
| Genotype | cdk9T120G::natMx dis2-11 ura4-D18 leu1-32 |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | 3MB-PP1 (10 microM) | -5 min | 0 min | 5 min |
| Pipeline info and software versions | |
| Sample notes | cdk9as strain is sensitive to 3MBPP1; dis2-11 strain is cold sensitive; 2 biotins used |
| Analysis notes |
| Sample QC score | 4 |
| Sample NRO score | 5 |
| Raw read length | 76 |
| Read depth after trimming | 12,874,948 |
| Duplication proportion | 0.86229 |
| Proportion reads mapped | 0.8477 |
| Exon/intron ratio | 9.2014 |
| Unique read proportion | 0.13857 |
| Total # Tfit bidirectionals | None |
| # Promoter Tfit bidirectionals | None |
| # Intronic Tfit bidirectionals | None |
| # Intergenic Tfit bidirectionals | None |
| Total # dREG bidirectionals | None |
| # Promoter dREG bidirectionals | None |
| # Intronic dREG bidirectionals | None |
| # Intergenic dREG bidirectionals | None |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |