DBNascent | Samples

SRR609665 Sample ID 2288

**Data sources**
Paper identifier	Sienski2012transcriptional
SRP accession	SRP016875
GEO accession	GSE41729
Samples	SRR609665

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	33 Mb
Stranded gene read counts	TXT file	731 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	cell line
Cell type	ovarian somatic cell
Clone/Individual
Strain
Genotype
Construct	siRNA GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	No protocol for knock-down, unclear if ds RNAi or siRNA plasmid
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	59,527,816
Duplication proportion	0.94346
Proportion reads mapped	0.4942
Exon/intron ratio	1.20291
Unique read proportion	0.1447

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False