DBNascent | Samples

SRR6492219 Sample ID 524

**Data sources**
Paper identifier	Cuartero2020control
SRP accession	SRP130916
GEO accession	GSE108584
Samples	SRR6492219

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	40 Mb
Stranded gene read counts	TXT file	925 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	530 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain
Genotype	Rad21 +/+; tamoxifen-inducible Cre/+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	hydroxytamoxifen (200 nM)	-72 hr	0 hr	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	hydroxytamoxifen induces nuclear translocation of ERt2-Cre to knock out floxed fragment
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	87,584,515
Duplication proportion	0.93719
Proportion reads mapped	0.6431
Exon/intron ratio	2.08916
Unique read proportion	0.25791

**Bidirectionals**
Total # Tfit bidirectionals	12217
# Promoter Tfit bidirectionals	4415
# Intronic Tfit bidirectionals	4170
# Intergenic Tfit bidirectionals	2362
Total # dREG bidirectionals	12316
# Promoter dREG bidirectionals	5999
# Intronic dREG bidirectionals	3308
# Intergenic dREG bidirectionals	2140
Part of Tfit master merge?	True
Part of dREG master merge?	True