DBNascent | Samples

SRR6492220 Sample ID 525

**Data sources**
Paper identifier	Cuartero2020control
SRP accession	SRP130916
GEO accession	GSE108584
Samples	SRR6492220

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	68 Mb
Stranded gene read counts	TXT file	915 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	712 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain
Genotype	Rad21 +/+; tamoxifen-inducible Cre/+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	hydroxytamoxifen (200 nM)	-73 hr	-1 hr	3 day
treatment	lipopolysaccharide (10 ng/mL)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	hydroxytamoxifen induces nuclear translocation of ERt2-Cre to knock out floxed fragment
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	50
Read depth after trimming	37,632,754
Duplication proportion	0.67568
Proportion reads mapped	0.7346
Exon/intron ratio	1.72421
Unique read proportion	0.5484

**Bidirectionals**
Total # Tfit bidirectionals	18855
# Promoter Tfit bidirectionals	5540
# Intronic Tfit bidirectionals	6926
# Intergenic Tfit bidirectionals	4895
Total # dREG bidirectionals	16432
# Promoter dREG bidirectionals	5322
# Intronic dREG bidirectionals	6156
# Intergenic dREG bidirectionals	3916
Part of Tfit master merge?	True
Part of dREG master merge?	True