DBNascent | Samples

SRR6492221 Sample ID 526

**Data sources**
Paper identifier	Cuartero2020control
SRP accession	SRP130916
GEO accession	GSE108584
Samples	SRR6492221

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	45 Mb
Stranded gene read counts	TXT file	923 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	1010 Kb
dREG bidirectional regions	BED file	464 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain
Genotype	Rad21 +/+; tamoxifen-inducible Cre/+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	hydroxytamoxifen (200 nM)	-78 hr	-6 hr	3 day
treatment	lipopolysaccharide (10 ng/mL)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	hydroxytamoxifen induces nuclear translocation of ERt2-Cre to knock out floxed fragment
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	92,262,328
Duplication proportion	0.94171
Proportion reads mapped	0.7395
Exon/intron ratio	1.89009
Unique read proportion	0.28119

**Bidirectionals**
Total # Tfit bidirectionals	11578
# Promoter Tfit bidirectionals	3971
# Intronic Tfit bidirectionals	4110
# Intergenic Tfit bidirectionals	2419
Total # dREG bidirectionals	10784
# Promoter dREG bidirectionals	5018
# Intronic dREG bidirectionals	3153
# Intergenic dREG bidirectionals	1873
Part of Tfit master merge?	True
Part of dREG master merge?	True