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SRR6492226 Sample ID 531
| Paper identifier | Cuartero2020control |
| SRP accession | SRP130916 |
| GEO accession | GSE108584 |
| Samples | SRR6492226 |
| QC score | 4 |
| Replicate | 1 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 50 Mb |
| Stranded gene read counts | TXT file | 916 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 1 Mb |
| dREG bidirectional regions | BED file | 487 Kb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | primary cell |
| Cell type | bone marrow derived macrophage |
| Clone/Individual | |
| Strain | |
| Genotype | Rad21 fl/fl; tamoxifen-inducible Cre/+ |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | hydroxytamoxifen (200 nM) | -73 hr | -1 hr | 3 day |
| treatment | lipopolysaccharide (10 ng/mL) | -1 hr | 0 hr | 1 hr |
| Pipeline info and software versions | |
| Sample notes | hydroxytamoxifen induces nuclear translocation of ERt2-Cre to knock out floxed fragment |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 4 |
| Sample NRO score | 1 |
| Raw read length | 50 |
| Read depth after trimming | 59,939,690 |
| Duplication proportion | 0.83271 |
| Proportion reads mapped | 0.5515 |
| Exon/intron ratio | 1.54387 |
| Unique read proportion | 0.40219 |
| Total # Tfit bidirectionals | 13484 |
| # Promoter Tfit bidirectionals | 3647 |
| # Intronic Tfit bidirectionals | 5282 |
| # Intergenic Tfit bidirectionals | 3375 |
| Total # dREG bidirectionals | 11291 |
| # Promoter dREG bidirectionals | 3966 |
| # Intronic dREG bidirectionals | 4088 |
| # Intergenic dREG bidirectionals | 2613 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |