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SRR6660226 Sample ID 1535
| Paper identifier | Link2018analysis |
| SRP accession | SRP131922 |
| GEO accession | GSE109965 |
| Samples | SRR6660226 |
| QC score | 2 |
| Replicate | 1 |
| Protocol | GRO-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 103 Mb |
| Stranded gene read counts | TXT file | 917 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 2 Mb |
| dREG bidirectional regions | BED file | 921 Kb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | primary cell |
| Cell type | bone marrow derived macrophage |
| Clone/Individual | |
| Strain | C57BL/6J |
| Genotype | |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | Kdo2-Lipid A (10 ng/mL) | -1 hr | 0 hr | 1 hr |
| Pipeline info and software versions | |
| Sample notes | |
| Analysis notes | needs samtools/1.10 and 60Gb memory for samtools step; Major adapter contamination for some libraries and some very low/no read depth samples; Ran qc redo nextflow |
| Sample QC score | 2 |
| Sample NRO score | 1 |
| Raw read length | 35 |
| Read depth after trimming | 49,240,473 |
| Duplication proportion | 0.63003 |
| Proportion reads mapped | 0.7834 |
| Exon/intron ratio | 2.04366 |
| Unique read proportion | 0.60804 |
| Total # Tfit bidirectionals | 24389 |
| # Promoter Tfit bidirectionals | 6869 |
| # Intronic Tfit bidirectionals | 9661 |
| # Intergenic Tfit bidirectionals | 5944 |
| Total # dREG bidirectionals | 21211 |
| # Promoter dREG bidirectionals | 5513 |
| # Intronic dREG bidirectionals | 8908 |
| # Intergenic dREG bidirectionals | 5421 |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |