DBNascent | Samples

SRR6687446 Sample ID 558

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession	GSE110464
Samples	SRR6687446

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	26 Mb
Stranded gene read counts	TXT file	906 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	658 Kb
dREG bidirectional regions	BED file	221 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LG100268 (100 nM)	-1 hr	0 hr	1 hr
treatment	interleukin-4 (20 ng/microL)	-25 hr	-1 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	36
Read depth after trimming	22,144,517
Duplication proportion	0.7967
Proportion reads mapped	0.6016
Exon/intron ratio	3.02016
Unique read proportion	0.23455

**Bidirectionals**
Total # Tfit bidirectionals	7776
# Promoter Tfit bidirectionals	2461
# Intronic Tfit bidirectionals	3086
# Intergenic Tfit bidirectionals	793
Total # dREG bidirectionals	5143
# Promoter dREG bidirectionals	2753
# Intronic dREG bidirectionals	1214
# Intergenic dREG bidirectionals	414
Part of Tfit master merge?	True
Part of dREG master merge?	True