DBNascent | Samples

SRR6780908 Sample ID 584

**Data sources**
Paper identifier	Danko2018dynamic
SRP accession	SRP081110
GEO accession	GSE85337
Samples	SRR6780908

**Technical details**
QC score	1
Replicate	4
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	109 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	632 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	CD4+ T cell
Clone/Individual	individual 4
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	ionomycin (1 mM)	-30 min	0 min	30 min
treatment	phorbol 12-myristate 13-acetate (25 ng/mL)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	76
Read depth after trimming	43,233,899
Duplication proportion	0.42604
Proportion reads mapped	0.6114
Exon/intron ratio	3.71289
Unique read proportion	0.71333

**Bidirectionals**
Total # Tfit bidirectionals	21842
# Promoter Tfit bidirectionals	10670
# Intronic Tfit bidirectionals	8421
# Intergenic Tfit bidirectionals	3056
Total # dREG bidirectionals	14453
# Promoter dREG bidirectionals	6845
# Intronic dREG bidirectionals	5871
# Intergenic dREG bidirectionals	1699
Part of Tfit master merge?	True
Part of dREG master merge?	True