DBNascent | Samples

SRR6825349 Sample ID 286

**Data sources**
Paper identifier	Bonelt2019precocious
SRP accession	SRP134925
GEO accession	GSE111692
Samples	SRR6825349

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	56 Mb
Stranded gene read counts	TXT file	916 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	646 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain	C57BL/6
Genotype	Ebf1 fl/+; Cd23-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting CD23+	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	50
Read depth after trimming	34,051,257
Duplication proportion	0.5879
Proportion reads mapped	0.5509
Exon/intron ratio	4.71956
Unique read proportion	0.46945

**Bidirectionals**
Total # Tfit bidirectionals	18408
# Promoter Tfit bidirectionals	7903
# Intronic Tfit bidirectionals	6004
# Intergenic Tfit bidirectionals	2124
Total # dREG bidirectionals	14925
# Promoter dREG bidirectionals	7031
# Intronic dREG bidirectionals	4326
# Intergenic dREG bidirectionals	1778
Part of Tfit master merge?	True
Part of dREG master merge?	True