DBNascent | Samples

SRR6837046 Sample ID 820

**Data sources**
Paper identifier	Fonseca2019diverse
SRP accession	SRP135713
GEO accession	GSE111855
Samples	SRR6837046

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	25 Mb
Stranded gene read counts	TXT file	900 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	645 Kb
dREG bidirectional regions	BED file	239 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	peritoneal macrophage
Clone/Individual
Strain	C57Bl/6EiJ
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	Kdo2-Lipid A (100 ng/mL)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	8,681,113
Duplication proportion	0.45094
Proportion reads mapped	0.6076
Exon/intron ratio	1.75
Unique read proportion	0.50906

**Bidirectionals**
Total # Tfit bidirectionals	7651
# Promoter Tfit bidirectionals	2810
# Intronic Tfit bidirectionals	2810
# Intergenic Tfit bidirectionals	1257
Total # dREG bidirectionals	5548
# Promoter dREG bidirectionals	2764
# Intronic dREG bidirectionals	1451
# Intergenic dREG bidirectionals	918
Part of Tfit master merge?	True
Part of dREG master merge?	True