DBNascent | Samples

SRR6904125 Sample ID 164

**Data sources**
Paper identifier	Aprile-garcia2019nascent
SRP accession	SRP136551
GEO accession	GSE112379
Samples	SRR6904125

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	57 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	738 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	States in notes that cycloheximide and NSC624206 were used as pretreatment for heat shock, but based on paper results section this seems to not be the case for the PRO-seq samples
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	75
Read depth after trimming	11,461,592
Duplication proportion	0.27708
Proportion reads mapped	0.9291
Exon/intron ratio	4.31638
Unique read proportion	0.71342

**Bidirectionals**
Total # Tfit bidirectionals	26885
# Promoter Tfit bidirectionals	15647
# Intronic Tfit bidirectionals	9341
# Intergenic Tfit bidirectionals	2744
Total # dREG bidirectionals	17020
# Promoter dREG bidirectionals	9962
# Intronic dREG bidirectionals	5385
# Intergenic dREG bidirectionals	1926
Part of Tfit master merge?	True
Part of dREG master merge?	True