DBNascent | Samples

SRR6904130 Sample ID 169

**Data sources**
Paper identifier	Aprile-garcia2019nascent
SRP accession	SRP136551
GEO accession	GSE112379
Samples	SRR6904130

**Technical details**
QC score	3
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	55 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat shock (43 Celsius)	-90 min	0 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes	States in notes that cycloheximide and NSC624206 were used as pretreatment for heat shock, but based on paper results section this seems to not be the case for the PRO-seq samples
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	75
Read depth after trimming	10,666,298
Duplication proportion	0.29075
Proportion reads mapped	0.9292
Exon/intron ratio	6.04717
Unique read proportion	0.68632

**Bidirectionals**
Total # Tfit bidirectionals	30060
# Promoter Tfit bidirectionals	14878
# Intronic Tfit bidirectionals	12164
# Intergenic Tfit bidirectionals	4008
Total # dREG bidirectionals	23997
# Promoter dREG bidirectionals	10669
# Intronic dREG bidirectionals	9728
# Intergenic dREG bidirectionals	3596
Part of Tfit master merge?	True
Part of dREG master merge?	True