DBNascent | Samples

SRR7184287 Sample ID 2845

**Data sources**
Paper identifier	Zhao2019myod
SRP accession	SRP148464
GEO accession	GSE114657
Samples	SRR7184287

**Technical details**
QC score	2
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	81 Mb
Stranded gene read counts	TXT file	922 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	C2C12
Clone/Individual
Strain
Genotype	MyoD -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	myotube differentiation protocol	-48 hr	0 hr	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled GRO-seq, but they used 'biotin-11-UTP'
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	3
Raw read length	50
Read depth after trimming	35,608,793
Duplication proportion	0.61775
Proportion reads mapped	0.8405
Exon/intron ratio	6.45068
Unique read proportion	0.43162

**Bidirectionals**
Total # Tfit bidirectionals	50954
# Promoter Tfit bidirectionals	11569
# Intronic Tfit bidirectionals	19865
# Intergenic Tfit bidirectionals	16818
Total # dREG bidirectionals	39451
# Promoter dREG bidirectionals	8085
# Intronic dREG bidirectionals	15984
# Intergenic dREG bidirectionals	12799
Part of Tfit master merge?	True
Part of dREG master merge?	True