DBNascent | Samples

SRR7290022 Sample ID 1991

**Data sources**
Paper identifier	Oh2021enhancer
SRP accession	SRP150232
GEO accession	GSE115603
Samples	SRR7290022

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	119 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	812 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct	siRNA Rad21

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Previous identifier until 6/16/21 was Oh2020unpublished, documentation reflects that

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	50
Read depth after trimming	43,708,325
Duplication proportion	0.53104
Proportion reads mapped	0.9186
Exon/intron ratio	3.0572
Unique read proportion	0.51146

**Bidirectionals**
Total # Tfit bidirectionals	23747
# Promoter Tfit bidirectionals	9704
# Intronic Tfit bidirectionals	9206
# Intergenic Tfit bidirectionals	4664
Total # dREG bidirectionals	18744
# Promoter dREG bidirectionals	7945
# Intronic dREG bidirectionals	7156
# Intergenic dREG bidirectionals	3885
Part of Tfit master merge?	True
Part of dREG master merge?	True