SRR7413428 Sample ID 985

Data sources
Paper identifier Hou2019paf1c
SRP accession SRP151141
GEO accession GSE116169
Samples SRR7413428
Technical details
QC score 1
Replicate 1
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end paired
Raw strandedness read1 forward
Reverse complemented? True
Mapped strandedness read1 forward
Timecourse True
Control/Experimental control
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 110 Mb
Stranded gene read counts TXT file 918 Kb
Unstranded bidirectional read counts TXT file 17 Mb
Tfit bidirectional regions BED file 2 Mb
dREG bidirectional regions BED file 588 Kb
All data files ZIP archive
Cellular details
Organism M. musculus
Sample type cell line
Cell type C2C12
Clone/Individual
Strain
Genotype Paf1 -/-; dox-inducible Paf1 exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment doxycycline (1 microg/mL) -3 day 0 day 3 day
Extended notes
Pipeline info and software versions
Sample notes cells either maintained in dox for whole experiment or withdrawn, set -3 days as start of experiment for both conditions
Analysis notes
Sample metrics
Sample QC score 1
Sample NRO score 2
Raw read length 35
Read depth after trimming 30,605,507
Duplication proportion 0.44388
Proportion reads mapped 0.7251
Exon/intron ratio 4.58729
Unique read proportion 0.50107
Bidirectionals
Total # Tfit bidirectionals 21554
# Promoter Tfit bidirectionals 7464
# Intronic Tfit bidirectionals 8037
# Intergenic Tfit bidirectionals 3257
Total # dREG bidirectionals 13622
# Promoter dREG bidirectionals 5080
# Intronic dREG bidirectionals 3994
# Intergenic dREG bidirectionals 2583
Part of Tfit master merge? True
Part of dREG master merge? True