SRR7413439 Sample ID 996

Data sources
Paper identifier Hou2019paf1c
SRP accession SRP151141
GEO accession GSE116169
Samples SRR7413439
Technical details
QC score 2
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end paired
Raw strandedness read1 forward
Reverse complemented? True
Mapped strandedness read1 forward
Timecourse True
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 90 Mb
Stranded gene read counts TXT file 914 Kb
Unstranded bidirectional read counts TXT file 17 Mb
Tfit bidirectional regions BED file 703 Kb
dREG bidirectional regions BED file 366 Kb
All data files ZIP archive
Cellular details
Organism M. musculus
Sample type cell line
Cell type C2C12
Clone/Individual
Strain
Genotype Paf1 -/-; dox-inducible Paf1 exogenous (stable)
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment DRB (100 microM) -5 min 0 min 5 min
treatment doxycycline withdrawal -3 day 0 day 3 day
Extended notes
Pipeline info and software versions
Sample notes cells either maintained in dox for whole experiment or withdrawn, set -3 days as start of experiment for both conditions
Analysis notes
Sample metrics
Sample QC score 2
Sample NRO score 2
Raw read length 40
Read depth after trimming 27,292,773
Duplication proportion 0.47597
Proportion reads mapped 0.7308
Exon/intron ratio 4.45552
Unique read proportion 0.44489
Bidirectionals
Total # Tfit bidirectionals 8248
# Promoter Tfit bidirectionals 2875
# Intronic Tfit bidirectionals 2685
# Intergenic Tfit bidirectionals 959
Total # dREG bidirectionals 8491
# Promoter dREG bidirectionals 2876
# Intronic dREG bidirectionals 2567
# Intergenic dREG bidirectionals 1864
Part of Tfit master merge? True
Part of dREG master merge? True